Molecular genetic background of haemophilia A patients with discrepancy between one-stage and two-stage factor VIII assays
نویسنده
چکیده
Correspondence to: Dr. Anna Pavlova Institut für Experimentelle Hämatologie und Transfusionsmedizin , Universitätsklinikum Bonn Sigmund-Freud-Str. 25, 53127 Bonn, Germany Tel. +49/(0)/228/28 71 97 11, Fax +49/(0)228/28 71 60 87 E-mail: [email protected] Hämostaseologie 2010; 30 (Suppl 1): S153–S155 Human blood coagulation factor VIII (FVIII) is an essential protein for the blood coagulation. Its physiological relevance is evident from the severe bleeding disorder haemophilia A associated with FVIII deficiency or dysfunction. The accurate measurement of FVIII coagulant activity (FVIII : C) in plasma is important for
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One-stage vs. chromogenic assays in haemophilia A.
Haemophilia A severity is closely correlated to the factor VIII (FVIII) activity, which can be measured in different ways. The original one-stage clotting assay is still the most widely used. The two-stage coagulation assay eliminated many of the drawbacks of the one-stage assay and was further developed into the chromogenic assay, a two-staged test with purified coagulation factors in the firs...
متن کاملDiscrepancy between one-stage and chromogenic factor VIII activity assay results can lead to misdiagnosis of haemophilia A phenotype.
UNLABELLED Severity of bleeding phenotype in hemophilia A (HA) depends on the underlying mutation in the F8 gene and, ultimately, on the concentration and functional integrity of the factor VIII (FVIII) protein in circulating plasma. Initial diagnosis for HA and monitoring of treatment is typically performed by measuring of FVIII activity by either one-stage assay or chromogenic assay. We revie...
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